Hydrocyclone Mammalian Cells

Use of Hydrocyclones for Mammalian Cell Retention ...

Sep 06, 2006· A hydrocyclone with a volume of 2.56 cm 3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply).

Hydrocyclones as cell retention device for CHO perfusion ...

In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the …

The Use of Hydrocyclones for Mammalian Cell Retention in ...

高达10%返现· A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes. The apparatus gave cell separation efficiencies as high as 99%. Due to the fact that a 5-L-bioreactor was used, a sub-optimal configuration of overflow and underflow diameters was used in order to obtain high cell viabilities combined with ...

Use of Hydrocyclones for Mammalian Cell Retention ...

A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow ...

Hydrocyclones as cell retention device for CHO perfusion ...

Mar 17, 2020· In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary (CHO) cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in ...

Application of Hydrocyclone for Cell Separation in ...

produced by mammalian cell systems. Mammalian cells have the advantage of precisely producing recombinant proteins with the same authentic protein folding structure as those produced in human bodies. Developments achieved in the different cultivation systems have accelerated the progress of mammalian cell culture.

Acoustic Settler, Centrifuge, Hydrocyclone - Animal Cell ...

Mar 17, 2020· In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in the ...

(PDF) Separation of CHO cells using hydrocyclones

Commercially available hydrocyclones not designed for mammalian cell separation, such as those from Sartorius BBI Systems, have been used for perfusion cultures with success [56].

Hydrocyclones as cell retention device for CHO perfusion ...

In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the …

Hydrocyclones as cell retention device for CHO perfusion ...

Mar 17, 2020· In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary (CHO) cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in ...

Use of Hydrocyclones for Mammalian Cell Retention ...

Aug 01, 2006· A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply). Cultures of BHK and HeLa ...

Application of hydrocyclones for continuous cultivation of ...

Nov 22, 2011· Hydrocyclones (HCs) have been recently extensively evaluated for their application in the separation of mammalian cells in perfusion bioreactors. The high centrifugal force derived within hydrocyclones is the key mechanism for cell separation inside such small-sized simple devices.

(PDF) Separation of CHO cells using hydrocyclones

Commercially available hydrocyclones not designed for mammalian cell separation, such as those from Sartorius BBI Systems, have been used for perfusion cultures with success [56].

Hydrocyclones as cell retention device for CHO perfusion ...

In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in the recirculation loop were evaluated in batch ...

MEETING ABSTRACT Open Access Application of …

From 22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies Vienna, Austria. 15-18 May 2011 Background Hydrocyclones (HCs) have been recently extensively evalu-ated for their application in the separation of mammalian cells …

(PDF) Separation of CHO cells using hydrocyclones ...

Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) By R. Medronho. A model for performance prediction of hydrocyclones. By R. Medronho. A new method for yeast recovery in batch ethanol fermentations: Filter aid filtration followed by separation of yeast from filter aid using hydrocyclones.

"Hydrocyclones for single-use perfusion application" by ...

Hydrocyclones (HC) are very compact devices that promote solid-liquid separation under the action of a centrifugal field. Despite the small size, HCs have a large processing capacity and do not suffer from clogging. Therefore, several publications explored HCs as a potential cell retention device in perfusion applications in the last 20 years, but limited to non-disposable lab-scale ...

Very High Density of CHO Cells in Perfusion by ATF or TFF ...

cells/mL in growing state and at high viability for more than 2 weeks. Finally, with the pres- ... widely used production systems for mammalian cells, per- ... Refine Technology), perfusion floating filter,4 hydrocyclone,9

Application of Hydrocyclone for Cell Separation in ...

The present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures. Firstly, experiments were carried out to evaluate the physical characteristics controlling the separation performance of the hydrocyclone as well as the effect of the different operating conditions and different hydrocyclone geometrical ...

A two-step microengineered system for high-density cell ...

Although it could be adapted to numerous cell culture applications, this system was optimized and tested for suspension-adapted Chinese Hamster Ovary (CHO) cells. As the first step of the cell retention system, a miniaturised hydrocyclone was developed that could separate the cells with macroscopic volume processing rates (~200 mL/min).

1. Curriculum Vitae Dr. Elsayed Ahmed Elsayed

Germany. Thesis title “Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Culture”. 1996 – 1999 M.Sc. Biochemistry, Faculty of Science, Cairo University, Cairo, Egypt. Thesis title “Microbiological and Biochemical Studies on the Production of Natamycin”.

New technologies can solve downstream bottlenecks ...

Aug 31, 2020· Jungbauer cited hydrocyclone separation as an example, explaining that although it is cheap, simple and well suited to continuous mode manufacturing, the high shear forces generated make it problematic. “The downside is the high shear which makes it less suitable for the separation of cells – especially mammalian cells.” Continuous solutions

Separation of CHO cells using hydrocyclones. - Abstract ...

Nov 14, 2007· Lübberstedt M, Medronho RA, Anspach FB, Deckwer WD (2000a) Separation of mammalian cells using hydrocyclones. In: Proceedings of biotechnology 2000—11th international biotechnology symposium and exhibition, vol 1. Dechema eV, Frankfurt am Main, pp 460–462

Application of hydrocyclones for continuous cultivation of ...

Nov 22, 2011· Hydrocyclones (HCs) have been recently extensively evaluated for their application in the separation of mammalian cells in perfusion bioreactors. The high centrifugal force derived within hydrocyclones is the key mechanism for cell separation inside such small-sized simple devices.

Application of Hydrocyclone for Cell Separation in ...

Sep 29, 2005· In the present work, the application of a hydrocyclone for cell separation in continuously perfused cultures of mammalian cells was investigated. The characterization of the hydrocyclone performance showed, that a pressure range of 0.85 to 1.40 bar is most suitable to achieve a reliable and stable separation process. Secondly, the effect of hydrocyclone operation on the cell …

MEETING ABSTRACT Open Access Application of …

From 22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies Vienna, Austria. 15-18 May 2011 Background Hydrocyclones (HCs) have been recently extensively evalu-ated for their application in the separation of mammalian cells …

Hydrocyclones as cell retention device for CHO perfusion ...

In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in the recirculation loop were evaluated in batch ...

(PDF) Separation of CHO cells using hydrocyclones ...

Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) By R. Medronho. A model for performance prediction of hydrocyclones. By R. Medronho. A new method for yeast recovery in batch ethanol fermentations: Filter aid filtration followed by separation of yeast from filter aid using hydrocyclones.

Separation of CHO cells using hydrocyclones ...

Nov 14, 2007· Hydrocyclones are simple and robust separation devices with no moving parts. In the past few years, their use in animal cell separation has been proposed. In this work, the use of different hydrocyclone configurations for Chinese hamster ovary (CHO) cell separation was investigated following an experimental design. It was shown that cell separation efficiencies for cultures of the wild …

Very High Density of CHO Cells in Perfusion by ATF or TFF ...

cells/mL in growing state and at high viability for more than 2 weeks. Finally, with the pres- ... widely used production systems for mammalian cells, per- ... Refine Technology), perfusion floating filter,4 hydrocyclone,9

1. Curriculum Vitae Dr. Elsayed Ahmed Elsayed

Germany. Thesis title “Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Culture”. 1996 – 1999 M.Sc. Biochemistry, Faculty of Science, Cairo University, Cairo, Egypt. Thesis title “Microbiological and Biochemical Studies on the Production of Natamycin”.

New technologies can solve downstream bottlenecks ...

Aug 31, 2020· Jungbauer cited hydrocyclone separation as an example, explaining that although it is cheap, simple and well suited to continuous mode manufacturing, the high shear forces generated make it problematic. “The downside is the high shear which makes it less suitable for the separation of cells – especially mammalian cells.” Continuous solutions

Application of Hydrocyclone for Cell Separation in ...

The present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures. Firstly,...

US6878545B2 - Method for separating viable cells from cell ...

Mammalian cells, insect cells, plant cells and microorganisms are small, and their density is close to that of water, being typically about 1.05 g/cm 3. For that reason, the view exists in the prior art that it is not possible to separate those cells from aqueous cell suspensions using hydrocyclones.

EP1280885B1 - Method for separating viable mammal or ...

cells hydrocyclone outlet diameter hydrocyclones Prior art date 2000-05-11 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Not-in-force Application number EP20010927844 Other languages German (de) French ...

The Application of Dielectric Spectroscopy and ...

May 07, 2015· the cells with all the essential nutrients and removing all the waste by-products. The cells are usually retained within the reactor by a cell separating device such as a spin filte r, cross flow filter, hydrocyclone or acoustic cell settler, Figure 1A. However, these cell separators represent an added cost, can gradually